 Anvio is an open-source software platform designed to facilitate comprehensive analysis and visualization of microbial genomes. It provides automated and manual characterization of metagenome sequences, as well as integration of other omics data such as transcriptomes and proteomes. The platform also enables users to explore temporal genomic changes within natural microbial populations over time. This article was authored by Amarat Aran, Oskin Ciezen, Christopher Quintz, and others.