 This research has demonstrated the potential of using nanoparticles derived from the fruit of perihoto, metanilla speciosa rianu. X-blum to reduce cholesterol levels. The results show that the optimal formulation of nanoparticles consisted of 0.2% chitosan, 0.1% sodium tripolyphosphate, not TPP, and a volume ratio of 5 to 1. These nanoparticles had an average diameter of 269.3 nanometers, a transmittance of 99.37% and a polydispersity index of 0.378. Furthermore, these nanoparticles were found to be effective at reducing cholesterol levels, with an estimated 50% reduction in cholesterol levels observed when tested against the extract of perihoto fruit. This article was authored by Fania Putri Lu-Herningtias, Risalela Vifta, Narsiromawadi, and others.