 This research examined the potential use of silver nanoparticles produced by a fungal species found in healthy refina sativus leaves for antimicrobial applications. The synth-sized silver nanoparticles were characterized using UVVis spectroscopy, FTIR, XRD, DLs, TEM, and AFM. The results showed that the silver nanoparticles had a spherical shape with an average particle size of 4 to 30 nanometers and were highly toxic against human pathogens. This suggests that the silver nanoparticles could potentially be used as effective antimicrobial agents. This article was authored by Ted Singh, Kumori Juti, Amar Patnaik, and others.