 In this study, we compared the efficacy of endobronchial ultrasound, guided transbranchial needle aspiration, EBU-STBNA, and endobronchial ultrasound guided transbranchial mediastinal cryobiopsy, TMC. We found that TMC had a higher diagnostic yield than EBU-STBNA, especially in cases of lymphoproliferative disorders and metastatic lymph nodes. Additionally, TMC was also faster and safer than EBU-STBNA, with no complications observed in any of the patients.