 This study developed an improved echotime independent technique for measuring transverse relaxation time of glutamate in the frontal cortex, where structural and functional abnormalities have been associated with psychiatric symptoms. The optimized four-flit angle measurements at 0°, 12°, 24°, and 36° with matched repetition time were used in 9 human subjects, 6F, 3M, 27 to 49 years old, at 7 Tesla. Longitudinal and transverse relaxation rates for glutamate were measured from a 2x2x2 cubic centimeters voxel placed in three different brain regions, gray matter-dominated medial prefrontal lobe, white matter-dominated left frontal lobe, and gray matter-dominated occipital lobe. The results showed that the scan time per voxel for measuring glutamate transverse relaxation was shortened by more than 50% compared to the original MRZSS technique. The glutamate T2 was found to be 117.5 ± 12.9 MS, mean ± standard deviation, N equals 9, 107.3 ± 12.1 N equals 9, and 124.4 ± 16.6 MS, N equals 8, in the medial frontal, left frontal, and occipital voxels, respectively. The improvements described in this study make the MRZSS technique a viable tool for reliably measuring glutamate relaxation from human subjects in a typical clinical setting and can be applied to characterize the intracellular environment of glutamatergic neurons in a variety of brain disorders. This article was authored by Ningxia Li, Linxing Li, Yan Zhang, and others. We are article.tv, links in the description below.