 Northern blotting. Northern blotting is another molecular biology technique which is used for detection of specific RNA sequence from RNA samples. The procedure how northern blotting is performed that is isolation of the messenger RNA then separation of RNA according to their size on the denaturing agarose gel. Once there is separation of the RNA with the help of agarose gel then there is a transfer of RNA to a solid spot, fixation of the RNA and then hybridization of immobilized RNA to probes complementary to the sequence of the interest. So this is a similar technique where in case of Northern blotting and in case of Southern blotting we see that the procedure in case of Northern and Southern is almost similar. Here we are working with the messenger RNA but in case of Southern we work with DNA. Removal of the probe molecules that are non-specifically bound to solid matrix detection, capture and analysis of an image of specifically bound probe molecules. Northern blotting is another molecular biology technique which is used to identify specific sequence of messenger RNA from samples of the messenger RNA.