 The 454-GSFLX sequencing technology has been found to have a mean error rate of 1.07%, which is significantly lower than the 3-6% error rate reported in earlier studies using the GS20 system. However, the error rate is not uniformly distributed throughout the genome, instead being concentrated in specific regions. This suggests that there are certain factors that contribute to the error rate, such as the presence of homopolymeric stretches, the position within the sequence, the size of the sequence, and the spatial location of the sequence on the plate. By taking these factors into consideration, it is possible to reduce the error rate further. This article was authored by Miloza Tibor, Ferrera Stephanie, Peck Nicholas, and others.