 Little is known about the synaptic organization of associative cortical structures such as the medial prefrontal cortex. Here, the authors used two-photon optogenetic stimulation to obtain a detailed cellular resolution map of functional synaptic connectivity of the mouse medial prefrontal cortex, finding unique spatial patterns of local circuit connectivity in neurons that project to the basal lateral amygdala. This article was authored by Jo of Prince, British Pottel, Matthews Mon, and others.