 This new technique, called fast cloning, is a highly simplified, reliable and efficient PCR-based cloning method which allows for the insertion of any DNA fragment into a plasmid vector or into a gene, cDNA, in a vector at any desired position. It requires fewer PCR cycles than traditional cloning techniques, eliminates the need for gel purification of the PCR product or linearized vector and does not require any specialized enzymes or cloning kits. Additionally, this method is highly effective and reproducible and can be used for chimera construction, insertion, and multiple mutations spanning a stretch of DNA up to 120 base pairs.