 Hi there, my name is Susanne and I'm from the University of Cologne in Germany. And today I will talk about our paper which only recently became editor's choice in Femmes microbiology ecology, where we investigated the phonology of grotesque and tree canopies. Tree canopies form the largest interface between the earth and the atmosphere, but worldwide more than 3 trillion trees each tree provides habitat for millions of microbial taxa. It can be presumed that the enormous habitat heterogeneity in combination with microclimatic fluctuations and seasons may play a crucial role for structuring microbial communities. However, we still lack in the basic understanding of the driving factors affecting microbial diversity in the canopy region. For our investigation, samples were taken from different microhabitats across the entire tree surface during spring and autumn season of two consecutive years. For our metagenomic approach, total DNA was isolated. This was followed by a PCR amplification with highly group-specific primers for two prominent groups of plant-associated protists. Afterwards, Illumina sequencing was conducted. Variance petitioning analysis of our data revealed that besides microhabitat and tree species identity, the respective seasons seems to affect community assembly in the canopy and ground habitats. Seasonal differences were particularly evident within communities dwelling the microhabitat of fresh leaves. Interestingly, these communities became highly specific for the respective autumn sampling, indicating a variable outcome of community assembly right after colonization due to priority effects. In addition, we were able to detect specific spring and autumn taxa, whereas usually highly whole-specific root parasites were exceptionally abundant across the entire canopy surface during autumn, pointing towards the hithero-unknown role of forest canopies as potential physical filters for air-dispersed protistin pathogens.