 In this study, deep sequencing was used to identify microRNAs, myrnas, in trifoliate orange, citrus trifoliata, a keyword stuck of citrus. The study revealed 10 novel myrnas and 42 highly conserved myrna families, suggesting that specific myrnas exist in C. Trifoliata Furthermore, these myrnas are likely to be involved in citrus growth, development, and response to disease. This study provides valuable information about the presence of myrnas in trifoliate orange, which can help us better understand the molecular mechanisms underlying citrus growth and development. This article was authored by Yu Huoping, Korya Nicholas, Zhong Changqing, and others.