 This study examined the use of pleurotus florida to produce silver nanoparticles. The nanoparticles were characterized by UV visible spectroscopy, field emission scanning electron microscopy, transmission electron microscopy, and atomic force microscopy. The particles were found to be crystalline in nature and covered with biomolecules on their surfaces. The nanoparticles showed excellent antimicrobial properties, and the synthetic process was environmentally friendly and simple, making it suitable for large-scale production. This article was offered by Ravishankar Bhatt, Raganand and Deshpande, Charinabasipha V. Ganachari, and others.