 The study investigated the antifungal properties of Chitosan and its derivatives with the aim of developing a natural alternative to synthetic chemicals. It was found that Chitosan nanoparticles prepared from different concentrations of low and high molecular weight Chitosan exhibited varying levels of antifungal activity against three fungi strains. Furthermore, it was observed that the antifungal activity of Chitosan nanoparticles was dependent on their particle size and zeta potential. This article was offered by Lingyanning, Norazium-Muhammadzin, Atif-Sawa, and others.