 move on we're going back to okay thank you you can stop sharing thank you very much okay now we're gonna we're gonna move back we're going back to Cape Coast uh so peter uh peter and a bunch of friends um are you there you can turn on your video and you can unmute hi can you hear me please yes okay yeah good evening to everybody um it was said we're dreaming from the University of Cape Coast as I've been already introduced I'm presenting on uh a technique that has helped us to safely discriminate cataract lens from healthy lens tissue of the sprak delirats and the basically these are my team members who help in the work and then this is my outline for the presentation introduction is from entameter and procedure and then I'll talk about some results and then conclusions cataracts as we all know is a major course of visual disability where what globally is a major course of visual disability and it accounts for more than half of blindness in developing countries and actually it increases absorption and then significantly decreases vision over time now contracts has been projected to increase globally due to increase in our aging population clinically of dream clinical experiments the good standard of evaluating cataract lens or discriminating cataract lens from healthy lens is history pathology history pathology has been the good standard of evaluating cataract lens from healthy lenses but this method has been found to be very very very subjective therefore there's the need for us to go get an objective technique that is also non-destructive to be able to discriminate cataract lenses from healthy lenses and then laser induced auto fluorescence technique with if advantage of being rapid and highly sensitive have been found to be a technique that is widely used in the medical field and then in the in the optometry automotive field it has also been used widely for research researchers have used it to actually monitor the healing process of conia they've also used it so many times and ma'am was love and his team have also used this same technique to work on secondary cataracts but interestingly they used tradition in the ultraviolet region but this tradition has also been found to cause cataracts long exposure of this tradition rather causes cataracts it's has another way of by which cataract is also being formed so it makes the usage of this tradition very on safe for the technique therefore we embark on analysis to use other techniques other licenses that can which are safe that can also be used to differentiate or discriminate cataract lens from healthy lens so the objective of the work is to use laser induced auto fluorescence technique with a safer light source to discriminate cataract lens from the healthy lens so experimentally 10-day old spagol rats were used of edas ex they were put into groups 45 per group one group was actually kept as the control and the other group was the cataract was actually created another group and then after cataract was developed the lenses in the the catalysis were actually removed and then it was integrated and was safe for measurements so this is the experimental setup the laser induced auto fluorescence setup that was used to acquire auto fluorescence from the lens tissues of both the healthy lenses and the cataract of lenses and it comprises of a laser source here as I said earlier on we use a cipher light light source in the visible region so 445 nanometer and then 405 nanometer light source was used comprises of microscope objective a fiber pod micro processor in the bifurated fiber and the Texas system is UV 2000 spectrometer so in acquiring the auto fluorescence from the from the lenses the lens the lenses were put on the sample stage and then auto fluorescence was applied from each of the lenses so this is a figure of normalized mean auto fluorescence spectrometer from both cataract lens and that of the healthy lens and as you can see it ranges a fingerprint standard from 450 nanometers to about 750 nanometers and the realize that the auto fluorescence from the cataract lenses are higher compared to that of the healthy lenses then employing principle component analysis we were able to discriminate the cataract lenses from the from the healthy lenses so here you can see that the red the red with the cataract lenses and the blue is the healthy lenses and we further classify new cataract lenses using employing facial linear discriminant analysis and employing this we're also able to classify a new lens as either being cataractors or a healthy lens so in all we are saying that the laser induced auto fluorescence technique has been able to have been used to discriminate cataract lenses from healthy lens and then cataract lenses show higher auto fluorescence intensity than that of the healthy lens and using principle component analysis three pcs were able to confirm the discrimination between the cataract lens and that of the healthy lenses and then employing the facial linear discriminant analysis we have hundreds of hundred percent success in discriminating cataract lenses from the healthy lenses so in all the citation laser source in the visible region offer a safer uh laser induced auto fluorescence technique to objectively distinguish and discriminate cataract lenses from the healthy lenses these are some references and then I thank you for your attention thank you very much thanks a lot Peter that was that was great um is this a low cost system can is it small enough can you take it to remote areas if you wanted to apply this to people not to rats yeah um uh system is actually relatively low cost uh as you said it's the it's relative depending on who is actually buying the or actually putting the setup together but in actual fact apart from the spectrum it is actually expensive I think it's quite expensive relatively low yeah okay thanks any other questions that don't look like it okay thanks a lot Peter I'll wait Nicoletta's got a question uh yeah hello uh very very nice work yeah I have a question it is a fluorescence spectrometer you use or you just use the normal spectrometer and you have some filters to block the question thank you for your question we use the normal spectrometer but we use the filter high pass filter to block the citation source okay okay great thanks all right great well thanks thanks a lot Peter say hi to everybody and thank you okay so next up Henry Henry year round