 Human intestinal organoids, HIOs, can be used as a source of intestine for regenerative therapies. However, HIOs require a suitable environment to grow and thrive. We found that HIOs seeded onto a cellular porcine matrices did not thrive in vivo, but HIOs seeded onto PGA-PLLA scaffolds did. This suggests that PGA-PLLA scaffolds may provide a more suitable environment for growing HIOs and could potentially be used to create a functioning human intestine. This article was authored by Stacey O'Finkbiner, Jennifer J. Freeman, Minna M. Week and others.