 O and Fe nanoparticles and their conjugates with the drug methotrexate were produced through a safe method of metal vapor synthesis, MBS. These materials were then analyzed using transmission electron microscopy, TM, X-ray photoelectron spectroscopy, XPS, and small-angle X-ray scattering, SA-XS, using synchrotron radiation, SA-XS. The use of acetone as an organic solvent in the MBS process allowed for the production of O and Fe nanoparticles with an average size of 8.3 and 1.8 nanometers, respectively. This was confirmed by TM Imaging. Additionally, the researchers observed that O, both in the nanoparticle form and when combined with methotrexate, was predominantly in the O0, O+, and O3+, states. The O4S spectrum for O-containing systems was similar, suggesting that the same mechanism was responsible for the formation of all three states. The researchers also noted that the addition of methotrexate caused a slight reduction in the proportion of the O0 state from 0.812. This article was authored by Alexander Vasilkov Anastasia Voronova, Tzvitilina Batsilova, and others. We are article.tv. Links in the description below.