 Spray bottles with 70% ethanol should be available in the cell culture room. Make sure that the spray bottle is properly labeled, and there are bottles dedicated to this room. You use ethanol to spray on gloves before working in the hood. Make sure you remove the extra ethanol off the glove, and that you do not do that when working with another plant flame. Ethanol is also used to clean items that will be brought inside the cell culture hood. Do not spray the items directly, but spray a tissue instead and use it to wipe the surface of bags containing cell culture items that then will be opened inside the hood. Take what you need from the bag and close it well.