 is an extremely common media formulation if you want to grow an E. coli or other bacteria in the lab. Albimedia is a very very common, cheap and cost effective way to grow microbes in the lab. Let's go to the recipe. Trypton is a digest of a protein known as Kezi that is most abundantly present in the mill. Trypton serves as a source of amino acid residues for bacteria. Next we have yeast extract. Yeast extract contains all of the nutrients required for the growth of bacteria. We have got anaceal microbes in particular use anaceal as a way to balance osmolytes in the medium means not too much salty, not too much wet. So in this experiment we will demonstrate the preparation of liquid albimedia to initiate bacterial culture in a shaking cloth. For the next step we need an analytical weighing balance. Place the weigh bar onto the balance pan. Share the key to zero out the weighing bar. Now use the lab scoop to add 1 gram of trypton, 1 gram of anaceal and 0.5 gram of yeast extract. This is 1 gram of trypton. Add 1 gram of anaceal, anaceal, 1 gram of trypton, 1 gram, 5 gram of yeast over the foil and label it with the meat in order to grow in large amounts. So first we will sterilize it by putting it on the sterilization done. This is a culture of E. coli. We will pick the colonies of the bacteria E. coli with the help of inoculating lube and we will mix it in a liquid broth media. After sterilizing the lube cool it for a bit and pick the colonies of the bacteria to the shaking incubator for overnight. Into the shaking incubator 37 degrees C for 24 hours. Off the shaking incubator and observe the flask. The solution which clearly shows the growth of the bacterial colony.