 The study presents a proof-of-principal demonstration of rapid-hole blood bioassays based on microwave-accelerated metal-enhanced fluorescence, MUMF, method using silver nanoparticle-deposited surfaces. Silver nanoparticles were deposited onto glass slides in a highly reproducible manner, and atomic force microscopy was used to determine their size. A model bioassay based on the interactions of biotinylated bovine-siramell-bueman, BBSA, and streptavidin was constructed on silver nanoparticle films. The model bioassay was run at room temperature for 60 minutes without microwave heating or with microwave heating for one minute. Whole blood samples could be used in MUMF-based bioassays, which had a lower detection limit of 0.01nm for BBSA compared to MEF-based bioassays that only allowed the use of samples in buffer solution and had a detection limit of 1nm for BBSA. This article was authored by Kedir Oslan.