 The technique of direct immunofluorescence, DIF, is essential in the accurate diagnosis of renal glomerular diseases. DIF can be performed on fresh frozen tissue or formalin fixed, paraffin embedded, FFPE, renal biopsy specimens. However, there are several methods available for performing DIF on FFPE samples, which have varying degrees of success. This study evaluated three such methods and compared their results with those obtained using DIF on fresh frozen tissue. The results showed that the immunofluorescence staining on the paraffin sections was less sensitive and less intense than DIF on fresh frozen tissue, but the diagnostic findings could still be detected in most cases. Therefore, it is possible to establish the diagnosis in most cases of immune complex mediated glomerular diseases with DIF on FFPE tissue specimens. This article was authored by Muhammad Mubarak, Javed Iqazi, Ami Kulsam, and others. We are article.tv, links in the description below.