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Published on Jan 25, 2013
(Presented in Mandarin) Visit http://www.bio-rad.com/yt/1/Multiplex... for additional background on the principles and techniques of real-time PCR. In this webinar, Dr Feng Pan presents the basic principles of quantitative PCR (qPCR) and discusses ways to optimize sensitivity and reproducibility in real-time PCR assays, both single-target amplification using SYBR® Green I and multiple-target amplification (multiplexing). Dr. Pan covers assay design, amplicon selection, secondary structure considerations, primer location and sequence, the use of melt curves to analyze amplification specificity, amplification efficiency, template DNA preparation, temperature optimization, and other topics.
He then introduces the concept of multiplexing in qPCR, touching on the various types of probes and primers available along with their benefits and the conditions to take into account when using them. He concludes with a brief treatise on data analysis for qPCR and addresses possible sources of error and reaction inefficiency. Every molecular biology laboratory can benefit from applying these methods to achieve better results.
Presenter: Feng Pan, PhD Field Application Specialist at Bio-Rad Laboratories
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