 Glutathione's physiological roles have expanded over the years from antioxidant defense and nutrient metabolism to regulating cellular functions such as gene expression, DNA and protein synthesis, signal transduction, cell proliferation, and apoptosis. This review focuses on Glutathione's role in cells signaling by analyzing its ability to modulate nitroxidative stress, autophagy, and viral infection. This article was authored by Katia Iacolano, Sarah Abaldelli, and Maria Rosa Isiriolo.