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HeLa cells bypassing mitosis to enter into senescence

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Uploaded on Dec 14, 2011

HTLV-1 Tax-expressing HeLa-FUCCI (fluorescent ubiquitin cell cycle indicator) cells bypass mitosis and become senescent. Ad-Tax-transduced cells were released from G1/S arrest and photographed every hour for 140 hours. The image of cells at the 140th hour is shown. Image processing software was used to move the Tax-expressing senescent cell (top) in close proximity to the normal growing colony (bottom). Cells with red, yellow, and green nuclei are in G1, G1/S, and S/G2 phases of the cell cycle respectively.

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