 And the first time we'll talk about simple, simple delusion, right? And on your lab there, we have a couple examples of a simple solution, right? If we took a solution and in there, in the example, we had two mils of solution that had 20 particles in it, so that it had 10 particles per mil, right? It had 20 per two mils, so it had 10 particles per mil, right? So if we take one mil and transfer this tube, we're taking 10 particles, right? And then you can't read that, but that's 10 particles, right? If we take one mil buffer as our diluent, then we're going to wind up with a total of two mils, right? That have 10 particles, so we have 10 particles per two mils or five mils. So we went from 10 per mil to 5 per mil, so our number was half, so we had one mil plus one mil, right? And so that was one mil now to two, one mil up to two mils, so it's one half. So our one half times 10 per mil is five per mil, so our dilution would be a one half of fractional form, so we multiplied our concentration initially, which was 10 particles per mil, then our resulting dilution gives us five particles per mil. So that's a simple dilution. If we did the same thing, we took a mil, let me come over here, there's our one mil in there and we put it in kind of a shaky looking tube, but we're going to add nine mils of buffer, right? So this one mil still came from there, so it has 10 particles in that one mil, so our total volume then is going to be 10 mils. So we have one out of the 10, so we've got one over one plus nine, right? So we've got one tenth, so we've done a one tenth dilution, so this 10 particles in 10 mils is a concentration of one particle per mil, right? So if we started with 10 per mil, multiplied by the dilution, then we wind up with the resulting concentration of one particle per mil. And as I said, particle, dilutions, it could be your concentration, your molar, it could be one molar going to 0.5 molar, going to one tenth molar. It could be milligram per mil, any kind of concentration, these could be cells, these particles could be one million cells per mil. But this is a notion of a simple dilution, you're taking something of a concentration in some, do you want some buffer and getting the resulting dilution of that.