 This study assessed the knowledge, perception and attitudes of parents towards lumbar puncture, LP in Riyadh, Saudi Arabia. It was found that 56.1 percent of respondents had a bad perception and 51.1 percent had poor knowledge about LP. Additionally, 70.4 percent had a positive attitude towards the procedure. Furthermore, there was a significant association between educational level and knowledge and perception of LP. In conclusion, it is important to target future research and campaigns towards improving the societal image of LP.