 The study identifies CLIP4 as a central node in the protease network involved in insect humoral immune responses, which is regulated by protease cascades and mosquitoes. CLIP4 is required for melanization and antibacterial immunity, acting as a prophenoloxidase, proprio, activating protease that is inhibited by SRPN2. The study also provides insight into the structural organization of the CSP network in Nganbi, demonstrating that CLIP4 activates ProClip8 and delivers positive reinforcement in proprio activation cascades. This article was authored by Shilfeng Zhang, Sha Sha Zhang, Junyao Kuang, and others.