 This study demonstrated the potential of furan for constructing bright NR2 fluorophores for biological imaging. Furan was used as the D unit to construct NR2 fluorophores, which resulted in red shift of absorption, enhanced absorption coefficient, and fluorescent quantum yield when compared with the ophen counterparts. These fluorophores were then applied for dual NR2 imaging of tumors and sentinel lymph nodes, LNs, enabling the in vivo imaging navigated LN surgery in tumor-bearing mice. This article was authored by Chun Chun Lu, Meng Feili, Huilong Ma, and others.