 This paper discussed the achievements of in vitro modeling of the blood-brain barrier, BBB, and provided an overview of the research area. It described the BBB's structure, function, and importance for the central nervous system, and outlined criteria for evaluating BBB models in vitro. It then discussed various techniques used to develop BBB models, including primary cultures versus cell lines, monocultures versus multicultures, and models on a chip, 3D models, and microfluidic models. Finally, it highlighted the potential benefits of these models for advancing neuroscience and the pharmaceutical industry. This article was authored by Andre Locke, Agnieszko Nuck, and Anna Katarzyna-Wadowitz.