 Miniaturization of diagnostic tools for biomolecular detection has become increasingly important due to its ability to reduce costs and increase efficiency. One such tool is nucleic acid amplification, which can be used to detect a limited amount of targeted molecules. Microfluidics are being used to miniaturize this process, allowing for faster and more accurate results with smaller sample volumes. Isothermal amplification methods, which do not require thermal cycling, are also being explored as alternatives to traditional PCR. These methods offer simpler designs and reduce complexity, making them ideal for miniaturizing nucleic acid amplification. This article was authored by Giuseppe Spotto and Laura Maria Zinoli.