 This scoping review aims to describe and categorize the most important barriers to guideline implementation and provide an overview of suitable strategies to overcome them. The search algorithm led to the identification of 69 articles which were analyzed using a qualitative synthesis approach. Barriers to guideline implementation can be differentiated into personal factors, guideline-related factors, and external factors. Successful strategies for guideline implementation include dissemination, education and training, social interaction, decision support systems, and standing orders. A structured implementation can improve adherence to guidelines, so analyzing barriers and adherence in advance is necessary to develop tailored strategies for specific settings and target groups. This article was authored by Florian Fisher, Kirsten Lange, Christina Close, and others.