 30 to 40 years of research has recognized the importance of studying microorganisms growing as Cecil communities in human infections but mimicking their biofilm lifestyle has been challenging. Models ranging from simple in vitro to complex in vivo have contributed to our understanding of biofilm physiology within a host context and promising preventive or curative strategies are emerging for biofilm related infections. This review analyzes the literature and discusses future venues and new approaches that can be merged with traditional techniques to model biofilm infections and fight them efficiently. This article was authored by Christof Beloyne, Alea Rendwails, David Lebeau, and others.