 The study aimed to identify micro-ionase, myrnas, that play a role in plant response to drought stress using high-throughput sequencing in Medecogo Trancacula, a legume model plant. 283 and 293 known myrnas were identified from the control and drought stress libraries, respectively. Additionally, 238 potential candidate myrnas were identified, with 14 new myrnas and 15 new members of known myrna families, whose complementary myrnas were also detected. Both high-throughput sequencing and RTQPCR confirmed that 22 members of four myrna families were upregulated, and 10 members of six myrna families were downregulated in response to drought stress. The known and predicted targets of the drought-responsive myrnas were found to be involved in diverse cellular processes in plants, including development, transcription, protein degradation, detoxification, nutrient status, and cross-adaptation. The findings are important for understanding the roles played by myrnas in response to abiotic stress, particularly drought stress, in plants. This article was offered by Zhao Minghui, Chen Lei, Wang Chinsua, and others. We are article.tv, links in the description below.