 The use of fungi as reducing agents in the biogenic synthesis of silver nanoparticles is attractive due to its ability to produce large quantities of proteins, high yields, easy handling, low toxicity, and improved stability and biological activity. Various species of fungus can be used to produce nanoparticles with different characteristics, and the synthesis mechanisms are not yet fully elucidated. The synthesis can be optimized by adjusting parameters such as temperature, pH, silver precursor concentration, biomass amount, and fungus cultivation time. Silver nanoparticles synthesized using fungi have potential for use in controlling pathogens with low toxicity and good biocompatibility, opening perspectives for future investigations concerning their use as antimicrobials in health and agriculture. This article was authored by Mariana Guilter Casagrande and Renata Dalima.