 Extracellular vesicles, EVs, have significant potential as diagnostic, prognostic, and therapeutic agents due to their molecular patterns on the surface revealing cell of origin and targeting capabilities. Cancer alters cellular and EV glycosylation patterns, enriching EV surfaces with glycone moieties that play versatile roles in cancer. Lectins, which recognize specific glycones, are powerful tools for detecting novel cancer biomarkers on EVs, and the past decade has seen an increase in published articles on lectin-based strategies for this purpose. This review explores the roles of EV glycosylation in cancer, and summarizes the potential of lectins and lectin-based methods for screening, targeting, separating, and identifying improved biomarkers from the surface of EVs. This article was authored by M. D. Kirul Islam, Ms. Bakhan, Kamlesh Gidwani, and others.