 RTQPCR is a sensitive and increasingly used method for gene expression quantification. However, it is important to note that the expression of commonly used reference genes can vary significantly in certain contexts. In order to overcome this issue, RAF genes was developed, which is a community tool designed to identify the best reference genes for specific experimental conditions. This tool was validated with RTQPCR experiments across several organisms, showing that the candidates proposed by RAF genes generally outperform commonly used reference genes. This article was authored by Blula Stefan, Kalei Jiva Luba, Vibru Feeb, and others.