 glycerol can be used as a replacement for organic solvents when preparing anionic liposomes for SiRNA delivery. This method is faster and safer than traditional methods, and it produces liposomes with high encapsulation efficiencies and low cytotoxicity. Furthermore, this method can increase the resistance of SiRNA against degradation, making it an ideal delivery system for nucleic acid drugs. This article was authored by Xiohan, Yanglu, Zhao Luoshu, and others.