 TTSEC is a powerful technique for monitoring rapid changes in transcription from enhancers and promoters during the immediate response of T cells to stimuli such as ionomycin and PMA. This method has been shown to have higher sensitivity than traditional RNASEC methods, allowing researchers to identify many new primary response genes. It also allows researchers to observe the simultaneous transcription of enhancers and their target genes, providing insight into how these two processes interact. Furthermore, this technique can be used to monitor the dynamic landscape of enhancers and their associated transcription programs during cellular responses and differentiation. This article was authored by Mogo Michel, Corina Demo, Benedict Zacker, and others.