 TiO2 nanoparticle, N-piece, biosynthesis is a low-cost, eco-friendly approach developed using the fungi aspergillus flavus TFR7. A significant improvement was observed in shoot length, 17.02%, root length, 49.6%, root area, 43.6%, and root nodule, 67.5%, chlorophyll content, 46.4%, and total soluble leaf protein, 94%. A possible mechanism has also been hypothesized. This article was authored by Ramesh Raliya, Pretin Bisvas, and J. C. Tareftar.