 Abstract acute kidney injury, A.K.I., is characterized by sudden and rapid decline in renal function due to various causes. It is associated with high morbidity, mortality, and cost of treatment. A.K.I. is strongly linked to damage to proximal tubules, leading to distinct patterns of gene expression and epigenetic alterations. These changes lead to structural changes in the nucleus of these cells, but these changes are difficult to observe under conventional microscopes. Recently, gray-level co-occurrence matrix, GLCM, and discrete wavelet transform, DWT, have been used to identify subtle nuclear morphological alterations associated with mild tissue injury in rodents. We found that mild ischemic A.K.I. is associated with reduced textural homogeneity of proximal tubule nuclei, as measured by GLCM and DWT energy coefficients. This article was authored by Igor Pantic, Yelena Kumik, Stefan Dugalik, and others.