 Hello, my name is Giovanna Toneguolo and I'm a research investigator at the University of Naples and a Change Advanced Biotechnology in Naples, Italy. Here, I've worked with a paper recently published on cytometry practice and titled Compilation of Two Floor-Based Imaging Metals to Measure Individual Diablast Cells, Area and Volume. In this work, we provide the first simultaneous area and volume measurements of diablast cells flowing in microcapillaries by using ice feed medium microscopy and quantitative data processing based on image analysis techniques. Through phase area measurements, which are lacking in the routine clinical tests, are of special interest being a potential diagnostic parameter about the cell deformability and aggregability. Glass cylindrical microcapillaries with an inner diameter of 10 and 50 micrometre were placed on the cover slip of a Plexiglass flow cell. A diluted red blood cell suspension was fed into the flow cell using flexible tubing connected to an input glass reservoir and was collected in an output reservoir. The total pressure drop across some microcapillaries can be taken as proportional to the distance between the liquid mini-sky and the two glass reservoirs. To measure individual red blood cell volume and surface area, two kinds of cell shape were considered. The bicon-claved disc shape, which is found when the cell is at rest or when it slowly flows in a tube three to four times bigger than cell size, referred to as unbounded flow regime. And secondly, the parachute shape, which is found when the cell flows in a capillary with a diameter comparable to its own size, referred to as confined flow regime. Concerning the unbounded flow condition, the experiments were carried out in a 50 micrometre capillary. In these conditions, a red blood cell takes its bicon-claved disc shape, its contour is discretized by image analysis to measure surface area volume. Concerning the confined flow, as happens in a 10 micrometre capillary, red blood cell volume and surface area is evaluated by regard in the cell as an axisymmetric solid of revolution around the x-axis. On the basis of comparison with Coulter cell analyzer data, we show that high-speed imaging of 10 micrometre capillary flow provides a reliable way of measuring red blood cell size parameters. In particular, measurements of red blood cell surface area, commonly lacking in multi-clinical tests, are also obtained. Unlike impedance measurements, our microfooding technique is well treated for monitoring and measuring individual red blood cell genetic parameters, such as volume and surface area at a single cell level. It does not require suspending cells in electrolyte solutions and being done in busy technique could be used to analyze images of flow in red blood cells from in the red blood cell element as well. Thank you for watching this video.