 The optimal conditions for producing polyhydroxybutyrate, pHB, from residual glycerol by bacillus megaterium LVN01 were determined through a combination of batch and fed batch cultures. Temperature, carbon to a nitrogen ratio, and fermentation time were found to be the main factors affecting pHB production. The highest pHB productivity was achieved at 30.8 degrees Celsius, 44.9 moles moly 1, and 39.9 hours. These conditions were also applied to fed batch cultures, which produced similar results. The DCW was approximately 1.9 GL1, with pHB productivities of 29.5 MgL1H1 and 35.6 MgL1H1 for bioreactors of 5L and 14L, respectively. Characterization of the pHB included nuclear magnetic resonance, Fourier transform infrared spectroscopy, differential scanning calorimetry, thermogravimetric analysis, and dynamic thermal gravimetry. This article was authored by Javier Ricardo Gomez Cardozo, Rodrigo Velasco Buccelli, Nefalia Maureen Pereja and others. We are article.tv, links in the description below.