 The present study demonstrated the potential of native plant extracts of jacand, polygonamplebium, litziglutinosa, and vanguria spinosis for the green synthesis of silver nanoparticles, AGNPS, followed by their characterization and antibacterial activity. The AGNPs were found to be highly stable and showed strong antibacterial activity against gram-positive and gram-negative bacteria. Furthermore, the protein-protein interaction study revealed that the AGNPS could affect the antioxidant enzyme systems of bacterial cells. This study suggests that the AGNPS synthesized from P. Polybium are more stable and may have prolonged antibacterial activity. This article was authored by Cole McAgee, Namrata Boggit, Mudhubala Kumari, and others.