 This study provides researchers with an easily accessible mRNA profile of the endogenous signaling repertoire of four commonly used cell lines for GPCR research. These cell lines include ATK293, ATT20, BV2, and N18. By measuring the mRNA levels of a comprehensive profile of non-chemo sensory GPCRs and over 100 GPCR signaling related gene products, researchers now have a better understanding of the potential interactions between GPCRs and those signaling proteins. Additionally, this data can help researchers choose the most appropriate cell line for studying GPCRs and related signaling proteins. This article was authored by Mackie Ken, Wajah Miller-James, Lopez Jacqueline, and others.