 This paper presents a novel approach to lipidomic profiling that utilizes hydrophilic interaction liquid chromatography, HILIC, and trapped ion mobility spectrometry, TIMMS, to identify and distinguish lipid isomus. The authors demonstrate how this method can be used to analyze lipid metabolism in both healthy and diseased states. This article was authored by Tien Shia, Feng Jiu, Dong Hui Zhong, and others.