 The study investigates the triacyliceral tag accumulation process in clamidominus reenhardty, a model green microlgar used for biodiesel production. The results show that tags are enriched in palmitic, oleic and linoleic acids and accumulate in oil bodies in response to nitrogen deficiency. Oil synthesis is maximal between two and three days following nitrogen depletion and reaches a plateau around day five. Blocking starch synthesis does not result in tag-over accumulation compared to their direct progenitor, the arginine auxotroph strained 330. The study also highlights the importance of using direct progenitors as control strains when assessing the effect of mutations on oil content and suggests the existence of complex interplays between oil synthesis, genetic background and stress conditions. This article was authored by Kariah Patrick, Nguyen Mai, Fesla Boris and others. We are article.tv, links in the description below.