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Published on Apr 6, 2011
( http://www.abnova.com ) - Besides using the laser scanner to capture the 2D gel electrophoresis image, researchers can also use a UV light box to visualize separated proteins on their 2D gels. Learn how to capture the image in this tutorial. Two-dimensional gel electrophoresis, (2-DE or 2D electrophoresis), is a form of gel electrophoresis commonly used to isolate the proteins in a sample for further characterization by mass spectroscopy. Mixtures of proteins are separated in the 1st dimension by their charge (isoelectric focusing (IEF)). Then in the 2nd dimension, proteins are separated by their size (molecular weight). More videos at Abnova http://www.abnova.com