 The study aimed to develop a hexatria-contain-loaded transethosome, HTCTS, for treating skin conditions caused by microbes. The HTCTS was created using the rotary evaporator technique and box-banking design, BBD. The optimal formulation with code F1 contained lipoid, MG, 90, ethanol, percent. 25, and Sodium Cholate, MG, 10. This formulation had the following characteristics, 183.9 nanometers, 0.262 mV, minus 26.61 mV, and 87.79 percent particle size, PDI, and entrapment efficiency. Additionally, the HTCTS was tested for its ability to release hexatria-contain, which was measured using confocal laser scanning microscopy, CLSM, dermatokinetics, and in vitro release. The results showed that the ideal formulation of the HTC-loaded TES had the following characteristics, 183.9 nanometers, 0.262 mV, minus 26.61 mV. This article was authored by Alhassan H. Aota, Sana Hashmi, Naseem Akhtar, and others.