 This study has developed an optimized acellular nerve allograft, ANA, which has been shown to possess improved characteristics compared to traditional ANA. This includes increased porosity, greater biocompatibility, and enhanced ability to support Schwann cell growth and migration. Additionally, it has been found to be capable of promoting axonal regrowth and myelin formation, resulting in improved functional recovery following peripheral nerve injury. This article was authored by Tien Hao Yu, Chang Ayo, Tian Rang Ayo, and others.