 This study aimed to develop a multi-epitope vaccine for monkeypox virus, MPXV. The research team first identified and characterized several epitopes on the MPXV surface proteins, then combined them into one vaccine construct. They also tested the construct stability and expression rate in E. coli bacteria. The results showed that the vaccine construct had good stability and high expression rates, suggesting it could be used as a safe and effective MPXV vaccine. Further testing is required before the vaccine can be approved for use. This article was authored by Samira Sanami, Shaheen Nazarian, Sajid Ahmad, and others.