 This research project aimed to use wastewater-based epidemiology to determine the trends of New Syria meningitis, NTS, infection in Gainesville, Florida. The results showed that NTS was present in the wastewater of the city throughout the study period, with higher levels detected in certain months. Additionally, culture-based methods were used to identify circulating serotypes that are responsible for both clinical and subclinical infections within the community. This article was authored by Andrew Rainey, Lisa E. Emerson, Mariela J. Edelman, and others.